The aim of REFBAR project is to create an international, intersectoral and interdisciplinary network to facilitate the exchange of staff to progress developments in eDNA metabarcoding and genetic reference library for freshwater plankton microalgae that can
significantly enhance data quality, data quantity and usability across aquatic ecosystems in Europe and beyond. The focus will be on
developing staff and partner skills in the areas of long-read sequencing, (meta)barcoding, and metadata standards coupled with
improved molecular bioinformatics techniques which in turn will offer more reliable DNA reference library for eukaryotic and
prokaryotic photoautotrophs. The consortium is comprised of an international network of beneficiaries and partners with
internationally-recognized track record on metabarcoding and world leaders in development of eDNA filters and portable next generation sequencing capabilities. The academic beneficiaries will benefit from gaining experience in the development of on-site
filtering of eDNA, third generation sequencing and bioinformatics. Industrial staff will benefit from bi-lateral exchanges from the
scientific domains of biosystematics and freshwater ecology that is necessary for developing innovative eDNA solutions from the first
sampling step to bioinformatics pipeline.
REFBAR Reinforcing network for enriched freshwater phytoplankton barcode library
(, 2025 - 2028, running project)
Coordinator or leader of the project
Tapolczai Kálmán

